miracidium of Megalodiscus temperatus stained with the vital stain, Nile blue sulfate.  Ducts of a penetration gland (DP), apical papilla (AP), apical gland (AG), nucleus of the penetration gland (NP), brain or ganglion (BR), secretory sac (SS), germ ball (GB) and cilia (CI).  This photo was included in this section to reveal the difference a stain technique can make.  1000x
 

miracidium of Megalodiscus temperatus with the vital stain Nile blue sulfate revealing the apical papilla (AP), cilia (CI), apical gland (AG), penetration gland (PG), brain or ganglion (BR), secretory sac (SS), nucleus of epidermal plate in the first row (NR-1), and germ ball (GB). This photo was included here to compare it to the protein-silver technique.  1000x